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The influence of iron on nickel accumulator charging
Mičák, Tomáš ; Čech, Ondřej (referee) ; Pléha, David (advisor)
This bachelor's thesis is dealing with a problematics of iron influence on charging of accumulator, more precisely on its cathode. The theory describes comparision of an accumulators at our market, important properties of Ni-Cd accumulators, their function principle, events happening during charge-discharge cycles of Ni-Cd cells and measuring method that will be used to verify of the iron influence. The practical part deals with the influence of iron on the development of oxygen during process of battery charging, on how iron affects the potential shift of oxygen gassing and how temperature affects this event.
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Analysis of embryotoxic effect of hydrocortisone using chick embryotoxicity screening test (CHEST).
Janíková, Michaela ; Peterka, Miroslav (advisor) ; Hovořáková, Mária (referee)
Cleft lip is one of the most common human birth deffects. Its etiopathogenesis is multifactorial and many aspects of its occurrence remain unknown in the fields of both genetics and teratology. One of the set of known negative external factors causing cleft lip is chemical hydrocortisone. Its effect on cell proliferation is highly heterogeneous and depends on attributes of a specific cell population. In this work we studied the cleft beak origin after the hydrocortisone treatment on the basis of Chick Embryotoxicity Screening Test (CHEST). Our main aim was to detect cell cycle changes in the chick frontonasal process after hydrocortisone injection via flow cytometry analysis. Hydrocortisone caused S phase arrest within a minor subpopulation of highly granular cells with specific cell cycle. This sensitive subpopulation was localized in the areas of previously defined proliferative centers within the frontonasal process using immunohistochemistry of frozen sections. Quantitative analysis of cells in these areas revealed significant decrease of M phase portion in the hydrocortisone treated samples in comparison with the control samples. The TUNEL staining of histological sections was used to determine the apoptotic rate in the frontonasal process. The comparison between the control and the...
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Analysis of embryotoxic effect of hydrocortisone using chick embryotoxicity screening test (CHEST).
Janíková, Michaela ; Peterka, Miroslav (advisor) ; Hovořáková, Mária (referee)
Cleft lip is one of the most common human birth deffects. Its etiopathogenesis is multifactorial and many aspects of its occurrence remain unknown in the fields of both genetics and teratology. One of the set of known negative external factors causing cleft lip is chemical hydrocortisone. Its effect on cell proliferation is highly heterogeneous and depends on attributes of a specific cell population. In this work we studied the cleft beak origin after the hydrocortisone treatment on the basis of Chick Embryotoxicity Screening Test (CHEST). Our main aim was to detect cell cycle changes in the chick frontonasal process after hydrocortisone injection via flow cytometry analysis. Hydrocortisone caused S phase arrest within a minor subpopulation of highly granular cells with specific cell cycle. This sensitive subpopulation was localized in the areas of previously defined proliferative centers within the frontonasal process using immunohistochemistry of frozen sections. Quantitative analysis of cells in these areas revealed significant decrease of M phase portion in the hydrocortisone treated samples in comparison with the control samples. The TUNEL staining of histological sections was used to determine the apoptotic rate in the frontonasal process. The comparison between the control and the...
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The influence of iron on nickel accumulator charging
Mičák, Tomáš ; Čech, Ondřej (referee) ; Pléha, David (advisor)
This bachelor's thesis is dealing with a problematics of iron influence on charging of accumulator, more precisely on its cathode. The theory describes comparision of an accumulators at our market, important properties of Ni-Cd accumulators, their function principle, events happening during charge-discharge cycles of Ni-Cd cells and measuring method that will be used to verify of the iron influence. The practical part deals with the influence of iron on the development of oxygen during process of battery charging, on how iron affects the potential shift of oxygen gassing and how temperature affects this event.
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